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Efficient Factorial Optimization of Transfection Conditions Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor. However, whether one is transfecting plasmid DNA or inhibitory RNAs, different cell lines can have significant differences in the ease of transfection or cellular survival. Determining the optimal plating conditions for high transfection efficiency and low cytotoxicity can be highly involved.
Getting Started with Kaluza: Data Scaling and Compensation Adjustment This application note will show you how to adjust the data scaling and fluorescence compensation of flow cytometry data in Kaluza* by using the Add All Plots function, Spillover and Logicle Sliders.

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