Aislamiento del tejido FFPE

Es necesario maximizar la integridad, el rendimiento y la pureza de las muestras FFPE para minimizar el riesgo de perder información genética crítica. FormaPure Total aísla el ARN y el ADN totales del a menudo complejo tejido incluido en parafina y fijado en formalina (FFPE), y proporciona a los investigadores soluciones flexibles y escalables que generan resultados congruentes al tiempo que conservan los ácidos nucleicos de mayor integridad para dar soporte a la sensibilidad mejorada de las aplicaciones de secuenciación de última generación (NGS).

FormaPure Total es uno de los tres kits de reactivos de la familia FormaPure, que también incluye FormaPure DNA para el aislamiento del ADN y FormaPure RNA para el aislamiento del ARN. Los kits de reactivos FormaPure son un sistema de una sola química diseñado y optimizado para utilizarse en la NGS y en otras aplicaciones posteriores, como qPCR/ddPCR/PCR.

FormaPure Total Workflow Steps

Genomics Products Sequencing RNA and DNA Tubes graphic

Características

FormaPure Total Products

Part Number Product Name Kit Size Format List Price Buy

96 Preps

Liquid

-

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50 Preps

Liquid

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Especificaciones del producto

Application Uses Nucleic acid sample prep, Total NA Isolation, Total NA Extraction, Total NA Sequencing
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Nucleic Acid Sample DNA, RNA
Automated Available Yes
Item Specifications Referenced C16675

FormaPure Total for RNA Extraction

NGS performance starts with RNA integrity. Fragment analysis shows FormaPure reagent kits retain the best possible RNA integrity even with 10+ year old tissue blocks.

 

 

FormaPure RNA isolates RNA with superior integrity. Fragment analyses were performed with RNA isolated with FormaPure RNA (blue traces) and an alternative method (orange traces) from four different FFPE sample types. All extractions were performed with one 10 μm curl. DV200 values are presented for each of the chromatograms. DV200 values represent the percentage of RNA fragments > 200 nucleotides.


FormaPure Total for DNA Extraction
Sample Tissue Results

 

FormaPure DNA maintains detectability of low-frequency variants
without introducing extraction based experimental bias. DNA
extracted using FormaPure DNA is suitable for the most stringent
downstream applications including NGS.

 

FormaPure DNA and FormaPure Total provide:

• Consistent & efficient extraction performance
• Simple workflow with minimal hands-on time
• Proven compatibility with NGS and other downstream applications
• Flexibility to accommodate sample input variation
• Best in class performance for yield, integrity, and purity

         Genomics Products Next Generation Sequencing Table

Documentación

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