Colorantes de polímero fluorescente SuperNova
SuperNova Violet Dyes: Next-Generation Polymer Dyes
Beckman Coulter Life Sciences introduces next-generation violet polymer dyes bringing cutting-edge science to clinical research. Supernova violet dyes are a novel range of polymer dyes that are brighter than conventional fluorochromes.
SuperNova violet conjugated antibodies not only deliver unique brightness for flow cytometry staining, but also generate limited non-specific staining through our proprietary formulation.
Figure 1. Absorption and emission spectra λexc = 405 nm of SNv428, SNv605, SNv650, and SNviolet 786 in PBS.
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SuperNova violet 428 dye
SuperNova violet 428 dye (SNv428), the first dye of the series, is optimally excited by the violet laser (405 nm) with an excitation maximum of 414 nm. It has emission peaks at 428 nm and can be detected using 450/50 bandpass filters or equivalent. It is among the brightest dyes excitable by the violet laser, and is therefore particularly suited for the assessment of dimly expressed markers.
SuperNova violet 786 dye
SuperNova violet 786 dye (SNv786) is a tandem polymer dye, derived from the core SNv428. Therefore, both share the same absorbance characteristics, with a maximum excitation at 414 nm. With emission peaks at 786 nm, it is optimally detected using the 780/60 nm bandpass filters of the flow cytometer.
SuperNova violet 605 dye
SuperNova violet 605 dye (SNv605) is a tandem polymer dye, derived from the core SNv428. Therefore, both share the same absorbance characteristics, with a maximum excitation at 414 nm. With emission peaks at 605 nm, it is optimally detected using the 610/20 nm bandpass filters of the flow cytometer.
SuperNova violet 650 dye
SuperNova violet 650 dye (SNv650) is a tandem polymer dye, derived from the core SNv428. Therefore, both share the same absorbance characteristics, with a maximum excitation at 414 nm. With emission peaks at 646 nm, it is optimally detected using the 660/10 nm bandpass filters of the flow cytometer.
SuperNova violet 428, v605, v650 and v786 dyes are also available through our LUCID Custom Design Services.
SuperNova violet tandem-dye technology
SuperNova violet 428 dye is a Beckman Coulter Life Sciences proprietary bright polymer dye that can be activated with amine for tandem dyes, followed by maleimide activation for tandem conjugates. Rigidity of our polymer dye structure helps to reduce rotational energy leading to brighter emissions. This, along with our exceptional manufacturing in proprietary formulations helps to achieve optimized FRET efficiency and increased stability.
Figure 2. SuperNova violet tandem dye technology.
Contact us to find out more about our SuperNova violet polymer dye conjugates.
For Research Use Only – Not for use in diagnostic procedures.
