Cell Line Development Products

Cell line development is the process by which the cellular machinery is co-opted to manufacture therapeutic biologics or other proteins of interest. Discover products for this pivotal process below. 


Products for Cell Line Development

Optima XPN ultracentrifuge

Optima Ultracentrifuges

 Our premier line of ultracentrifuges for high quality separations
valitatiter igg quantification assay kit val003

ValitaTiter Assays

 Rapid, high-throughput IgG quantification in crude cell culture samples

CytoFLEX SRT

Simplify your population sorting with the CytoFLEX SRT Cell Sorter

Biomek i-Series Automated Liquid Handlers: Biomek i5 and Biomek i7

Biomek i-Series

Automate every step of the complex Cell Line Development workflow - from transfection to enrichment to limiting dilution to culture/feeding to assaying to hit-picking and expansion.

Echo 525 Acoustic Liquid Handler

Echo 525

Acoustic liquid handling for precise and accurate low volume transfers enabling assay miniaturization

Access Solutions Systems

 Discover a comprehensive suite of high-throughput automation platforms that are easily integrated to meet each critical step of your cell line development workflow.
Avanti JXN 26 high speed centrifuge

Avanti JXN Series

 High performance centrifugation for cell culture harvest and purification optimization

MoFlo Astrios

Provides an automated, high throughput single cell depositing system. This process is mandatory for master cell bank generation.

Vi-CELL MetaFLEX bioanalyte analyzer evaluates pH, pO2, pCO2, glucose, lactate, electrolytes and more

Vi-CELL MetaFLEX

 Use the Vi-CELL MetaFLEX for fast bioreactor media analysis

Vi-CELL BLU

Used at multiple steps along cell line development. Eliminate human error and user bias when counting cells and determining viability.

Avanti J-15R benchtop centrifuge

Avanti J-15R

Separate cells from culture media for antibody titer assays, quantification. Pellet cells for media exchanges.

Helpful Links

Content and Resources

Efficient Factorial Optimization of Transfection Conditions Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor. However, whether one is transfecting plasmid DNA or inhibitory RNAs, different cell lines can have significant differences in the ease of transfection or cellular survival. Determining the optimal plating conditions for high transfection efficiency and low cytotoxicity can be highly involved.
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR This application note details how you can use the plate loader feature on the Vi-CELL BLU to match cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
Considerations of Cell Counting Analysis when using Different Types of Cells This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer This application note details one of the key strengths of the Vi–CELL BLU, the ability to fine tune the performance to minimize variability between different instruments. This is particularly valuable in facilities that utilize multiple instruments but can also be important when instruments are occasionally shared between departments.
Cell Line Development – Limiting Dilution Limiting dilution is a traditional approach to achieve monoclonality. It entails diluting cells to the level that there is one cell on average per unit of volume plated in the wells. However, as predicted by the Poisson distribution, roughly one third of the plated wells will have zero cells, one third of wells will have a single cell, and one third will have multiple cells per well (Coller and Coller 1986).